High Resolution Liquid Chromatography Mass Spectrometer (HR-LCMS)
[Xevo QToF, Waters, USA]

Located at: CRF Annexe - G01
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• Settable flow rate range: 0.010 to 2.000 mL/min, in 0.001 mL increments

• Operating pressure: 15,000 psi up to 1.0 mL/min, 9000 psi up to 2.0 mL/min

• pH range: 2 to 12

With its flow through needle injector and robust, quaternary solvent delivery system, the ACQUITY UPLC H -Class PLUS Bio System has been specially engineered with a bio-inert flow path and Auto-Blend Plus Technology to address your most challenging bio separations. The complete flow path is engineered with iron-free, Bio inert materials, helping your laboratory extend the lifetime of your instrument and maintain optimal bio separations performance. The flexibility of multi-solvent blending with the Quaternary Solvent Manager (QSM), and blend four solvents in any combination or proportion. High-precision injections with direct inject sampling. The needle-in-flow path design ensures an accurate needle seal at high pressure. Injection volumes from 0.1 μL up to 1 mL are supported.


The ACQUITY UPLC H-Class PLUS Bio System provides the same high resolution, high sensitivity separations you have come to expect from UPLC Technology, with the flexibility and ruggedness to run all the chromatographic modes one can require:

Hydrophilic interaction (HILIC), or reversed-phase – all on the same system.

• Flow accuracy: ±1.0% at 0.5 to 2.0 mL/min using 100% A (with i2 Valve).

• Flow precision: 0.075% RSD or ±0.020 min SD, based on six replicates (with i2 Valve).

• Composition accuracy: ±0.5% absolute (full scale) from 5% to 90% from 0.5 to 2.0 mL/min (with i2 Valve).

Degassed ACN/H2O (90:10), ACN/H2O (90:10) with caffeine at 12 mg/L concentration, backpressure 2000 psi, step gradient method, UV at 273 nm

• Composition precision: < 0.15% RSD or ±0.04 min SD, based on six replicate injections Compressibility compensation: Automatic and continuous

• Priming: Wet priming can run at flow rates up to 4 mL/min

• Pump seal wash: Equipped with an automated wash system to flush the rear of the high pressure seal and the plunger

• Flow ramping Range: 0.01 to 30.00 min to reach 2.00 mL/min

• Default: 0.45 min to reach 2.00 mL/min

Specification of MS

Mass Range: The instrument has a high mass filter (quadrupole) for efficient transmission of ions having mass range atleast ≤ 30 to ≥ 32,000 m/z in resolving mode & ≤ 30 to ≥ 1,00,000 m/z in non-resolving mode. The mass range of the Time of Flight analyser is ≤ 30 to ≥ 1,00,000 m/z.

The resolution of the TOF mass analyser is more than 40,000 FWHM between m/z 950-960 and more than 35,000 FWHM at around 200 m/z. The mass accuracy of the system is minimum of less than 0.5 ppm, with both internal & external calibration standards for both MS & MS/MS modes on 10 consecutive repeat measurements on column analysis.


Metabolic phenotyping of cells/tissues

Identification and quantification of organic & chiral chemicals

Detection of API in liquid/solid samples

Micro pollutants detection

Detection of cancer markers

Detection of alkaloids in plant extracts

Extractable and leachable screening in e-cigarettes, food, cosmetics, and pharmaceutical packaging

Proteomics and protein sequence identification and analysis

Detection of chemical contaminants

Detection of Pesticides in Drinking Water

Testing charges per sample

NITK # Academic & Research Institutes #* Industries #*
UPLC Rs. 650/- Rs. 950/- Rs. 1900/-
UPLC-MS [For any one mode] Rs. 900/- Rs. 1350/- Rs. 2700/-
UPLC-MS-MS [For any one mode] Rs. 1400/- Rs. 2100/- Rs. 4200/-
MS [For any one mode] Rs. 550/- Rs. 850/- Rs. 1700/-
MS-MS [For any one mode] Rs. 900/- Rs. 1350/- Rs. 2700/-

# Compound identification and analysis charges are Rs. 1000/- per hour. Sample preparation charges are extra (charges will be calculated after discussions about sample preparation procedure)

* GST(18%) extra.

For further Query Contact:

Mrs. Thrithila Shetty (Technical Staff, CRF), Email: crf_hrlcms@nitk.edu.in

Dr. Keyur Raval (HR-LCMS Faculty in-charge), Email: keyurnraval@nitk.edu.in