Cell Imaging Multi-Mode Reader, [CYT5MPV, BioTek Instruments, USA]

Located at: CRF Annexe - S01
  • Images

Facilities: Module supports detection modes: UV-Vis absorbance, Fluorescence Luminescence, Time-resolved fluorescence, Read methods: Endpoint, kinetic, spectral scanning, well area scanning.

Applications: Absorbance, fluorescence and luminescence-based endpoint and kinetic assays, cell imaging: 6- to 1536-well plates.

Technical specifications Cell imaging multimode reader

• Detection mode: Fluorescence, Luminescence, UV-Vis absorbance and Time-resolved fluorescence

• Light sources: Xenon flash

• Detector: PMT for florescence system, Photodiode for absorbance

• Wavelength range: 250-700nm (fluorescence), 230-999nm (absorbance)

• Dynamic range: 7 decades (fluorescence), 0.4 OD (absorbance), >6 decades (luminescence)

• Reading speed (kinetic): 96 wells: 11 seconds, 384 wells: 22 seconds

• Assay type: End point, kinetic, spectral and well scanning read

• Plate compatibility: Reads 6 to 384 well plates and imaging of 6 to 1536 well plates. we can accommodate microscope slides, petri and cell culture dishes, cell culture flasks (T25)

• Plate shaking: Linear, orbital, double orbital shaking modes

• Imaging modes: Fluorescence, bright-field, color bright-field and phase contrast with single color, multi-color, montage, time lapse and z-stacking

• Image filter cube: DAPI, GFP, Texas Red Filter cubes with imaging LED cubes of 365nm, 465nm and 590nm

• Monochromator wavelength accuracy and repeatability: 2 nm, +0.2 nm, OD accuracy: <1% at 2.0 OD <3% at 3.0 OD, OD linearity; <1% from 0 to 3.0 OD, OD repeatability: <0.5% at 2.0 OD

Testing charges.

Plate reading assay [per sample/plate] Cell imaging [per sample per mode]
NITK Rs. 250/- Rs. 500/-
Academic Institute* Rs. 550/- Rs. 800/-
R&D Institute* Rs. 650/- Rs. 1000/-
Industry* Rs. 750/- Rs. 1200/-

*Sample preparation charges, GST(18%) extra. Above charges are for providing raw data.

For further Query Contact:

Dr.(Ms) Gangamma S (Cell-Imaging Faculty in-charge), Email: gangamma@nitk.edu.in, Mob: +91-8970110470